Bazı yabani mantarların antioksidan özellikleri

Abstract

Çalışmada Türkiye’de farklı bölgelerden toplanan 12 yabani mantarın (Lactarius piperatus, Tricholoma anatolicum, Amanita caesarea, Lactarius delicious, Lactarius sanguifluus, Cantharellus cibarius, Hydnum repandum, Picoa lefebvrei, Ramaria aurea, Lactarius semisanguifluuss, Craterellus cornucopioides, Laccaria laccata) antioksidan aktiviteleri ve özellikleri incelenmiştir. Toplanan mantarların methanolde elde edilen ekstrelerinde toplam fenolik madde miktarı Folin-Ciocalteu yöntemi, flavonoid miktarı kolorimetrik olarak, antioksidan yöntemi 2,2-Diphenyl-1-picrylhydrazyl (DPPH) yöntemi ile trolox eşdeğeri olarak belirlenmiştir. Mantarların toplam fenolik madde içeriği 575.10-2156.40 mg GAE/100g DW, flavonoit miktarı 103.01-346.53 mg CE/100g DW ve antioksidan aktivitesi 525.32 to 1693.85 µmol (TE)/100 g DW arasında değişmiştir.

The antioxidant activity and properties of 12 wild mushrooms (Lactarius piperatus, Tricholoma anatolicum, Amanita caesarea, Lactarius delicious, Lactarius sanguifluus, Cantharellus cibarius, Hydnum repandum, Picoa lefebvrei Ramaria aurea, Lactarius semisanguifluuss, Craterellus cornucopioides, Laccaria laccata) collected from Turkey were evaluated. Their methanolic extracts were used to determine antioxidant capacity (TAC), total phenolics and flavonoids. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were measured to evaluate antioxidant capacity of the extracts and expressed as trolox equivalents (TE). The amount of total phenolics was determined by using Folin–Ciocalteu method and Flavonoid contents in the extracts were determined by a colorimetric method. Wild mushrooms were found to be high in antioxidant phytochemicals, such as phenolics (575.10- 2156.40 mg GAE/100g DW), flavonoids (103.01-310.89 mg CE/100g DW). The TAC values of the spices ranged from 525.32 to 1693.85 µmol (TE)/100 g DW.