Fluorescent Copper Phosphate Nanoflowers: A Novel Toxicity Investigation Study Based On Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) Larvae

Abstract

Organic-inorganic hybrid nanoflowers with commercialization potential are the novel materials and are widely used in many experimental applications, recently. However their potential toxicity values of these nanoflowers are still unknown. In this study, fluorescent copper phosphate nanoflowers (FCPnfs) were first synthesized to constructed via organic-inorganic hybrid nanoflower synthesis method at Science Technology Application and Research Center Laboratory of Nevsehir Haci Bektas Veli University in 2018. The morphological features of FCPnfs (acridine orange amount, shape, size) were analyzed. The results showed that different amount of acridine orange caused different morphology and the organic component were homogeneously distributed inside the nanoflowers. These materials were characterized by SEM, EDX, XRD, and FTIR techniques. The toxic effects of the as-prepared FCPnfs were investigated in Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) larvae compared to pure copper phosphate nanoflowers (CPnfs) at Plant and Animal Production Department Laboratory of Nevsehir Haci Bektas Veli University, Avanos Vocational School in 2018. Nanoflowers were force feed to larvae at the different doses of 0.001, 0.005, 0.010, 0.025, 0.050, 0.100 mg/10μl. According to probit assay, LC50 and LC99 values of FCPnfs were found 0.490 and 0.145 mg/10μl, respectively. On the other hand, LC50 and LC99 values of CPnfs were detected 0.066 and 0.172 mg/10μl, respectively. It was found that the insect exhibited slightly more resistance to CPnfs than FCPnfs when compared to both chemical toxic values. These new findings will be offer a new insight into how to design new materials in industrial uses.