Abstract:
Sampling of salivary cortisol and amylase is a non-invasive method and important for
the evaluation of the hypothalamic–pituitary–adrenal axis function and stress levels. This
study aimed to compare the values of the salivary cortisol and amylase levels which were
measured by three diferent analytical methods to discuss the alterations of stress levels of
samples. The saliva samples of young adults (n=23) were collected between 08.00 and
09.00 a.m., noon at 12.00 (before exam) and between 14.00 and 15.00 p.m. (after unaware exam). The samples were measured within the frst 48 h, and no freezing/thawing
was done. Salivary cortisol and amylase levels of subjects were measured by three diferent
analytical methods as ELISA, chemiluminescence and biosensor methods. Comparison of
ELISA and biosensor methods in order to determine the salivary cortisol levels showed
a good correlation y=2.971+0.748x (R2=0.839). Salivary amylase concentrations were
only detected by ELISA method. Biosensor can be ofered as an alternative analytic
method to the conventional determination method ELISA. It can be preferred because of
the detection/information efectiveness, low cost, fast results and specifcity characteristics.