Abstract:
In this study, we aimed to investigate the changes in the malondialdehyde (MDA) content and cellular H2O2 accumulation, which are
the oxidative stress markers, and the expression levels of stress-related proteins such as metallothionein (MT) protein, catalase (CAT),
Superoxide dismutase (Cu/Zn-SOD), and glutathione S transferase (GST) enzymes. For placing oxidative stress on plants, we used Cd
in different concentrations (50,100 and 200 μM) and durations (12,24 and 48 h). The results revealed that MDA content and H2O2
accumulation linearly increase with exposure time and concentration. Although the Cu–Zn/SOD and MTexpressions increased at 24 h
exposure, they decreased at 48 h exposure. The GST gene is highly expressed at the beginning level of oxidative stress, whereas a
decrease in the level of expression was detected with increase in exposure time. Statistical significance was detected in CAT gene
expression at 12 h application; however, a decrease in expression level was detected in the 24 and 48 h applications. The results of this
study show that transcript accumulation of stress-related genes might coincide with the oxidative levels in the cell and the H2O2
concentration in the signalization structure.Moreover, it could accelerate the expression of the stress genes up to a certain concentration
degree, whereas a greater concentration of H2O2 could reduce transcript accumulation.
